"Israel is doing its very, very best to make sure we don't get out of port," spokeswoman Greta Berlin said, speaking by telephone from Greece where some of the ships are moored.
"We want to move the boats by July 5 to get to our rendezvous point no later than July 6 or 7 ... We will go with what we have," she added.
The small flotilla, including boats from the United States, France and Canada, was due to leave at least a week ago, but the departure has been constantly put back amidst allegations of a dirty tricks campaign to stop the pro-Palestinian initiative.
Israel says its blockade of Gaza is aimed at stopping weapons from reaching the enclave's rulers, Hamas -- an Islamist group which is branded a terrorist organization by the West.
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